1. Preparation

• Sanitize: Clean the patient’s facial skin and blood draw site (arm) with antiseptic.
• Gather Supplies:
  • Dermaheal ACD+GEL+Peptides tube(s).
  • Sterile butterfly needle, syringe, and tourniquet.
  • Centrifuge (PRP-optimized settings).
  • Microneedling device or mesotherapy gun (optional).

2. Blood Collection

1. Draw Blood:
   • Collect 8–10ml of venous blood directly into the Dermaheal tube.
   • The ACD Anticoagulant prevents clotting while preserving platelet integrity.
2. Gentle Mixing:
   • Invert the tube 5–6 times to blend blood with ACD and peptides. Avoid shaking.

3. Centrifugation

1. First Spin:
   • Centrifuge at 1500–2000 RPM for 10 minutes.
   • The GEL barrier separates RBCs (bottom) from PRP (middle) and platelet-poor plasma (PPP, top).
2. Second Spin (Optional):
   • Transfer the PRP layer to a sterile tube.
   • Spin at 3000 RPM for 5–8 minutes for higher platelet concentration.

4. PRP-Peptide Activation

• Extract PRP: Use a sterile pipette to collect the PRP layer (middle).
• Peptide Synergy: The pre-loaded peptides (e.g., Palmitoyl Tripeptide-1, Acetyl Hexapeptide-8) bind to PRP, boosting collagen synthesis and cellular repair.

5. Application to Face

• Microneedling:
  • Apply PRP after microneedling (0.5–1.5mm depth) for deep absorption.
  • Use a derma roller or automated pen.
• Mesotherapy:
  • Inject PRP intradermally into target areas (cheeks, forehead, nasolabial folds).
  • Use a 30–32G needle for precision.
• Topical Boost:
  • Mix PRP with hyaluronic acid serums or apply post-laser for enhanced results.

6. Post-Treatment Care

• Advise the patient to:
  • Avoid sun exposure, makeup, or harsh skincare for 24 hours.
  • Use gentle, hydrating products (e.g., ceramide creams).
  • Schedule 3–4 sessions (4–6 weeks apart) for optimal results.

ACD + GEL