1. Preparation

• Sterilize: Clean the patient’s skin (draw site) with alcohol or antiseptic.
• Equipment Ready:
  • Dermaheal ACD+GEL+BIOTIN tube(s).
  • Sterile blood collection kit (needle, syringe).
  • Centrifuge (calibrated for PRP protocols).
  • PPE (gloves, mask

2. Blood Collection

1. Draw Blood:
   • Insert the needle into the patient’s vein (typically antecubital vein).
   • Draw 8–12ml of whole blood directly into the Dermaheal tube.
   • The ACD (Anticoagulant) in the tube prevents clotting.
2. Gently Invert:
   • Tilt the tube 5–6 times to mix blood with the ACD and BIOTIN additives.
   • Avoid shaking to prevent platelet activation.

3. Centrifugation

1. Load the Tube:
   • Place the tube in a centrifuge, balancing it with another tube of equal weight.
2. Spin Settings:
   • First Spin:
     • Speed: ~1500–2500 RPM (varies by centrifuge model).
     • Duration: 5–10 minutes.
     • Goal: Separate RBCs (bottom) from plasma (top) using the GEL barrier.
   • Second Spin (Optional for higher platelet concentration):
     • Transfer the platelet-rich plasma (PRP) to a sterile tube.
     • Spin again at higher speed (3000 RPM) for 5–8 minutes.

4. PRP Extraction

1. Post-Centrifuge:
   • The tube will show 3 layers:
     • RBCs (bottom, trapped by GEL).
     • PRP + BIOTIN (middle).
     • Platelet-Poor Plasma (PPP, top).
2. Extract PRP:
   • Use a sterile pipette to collect the PRP layer (middle).
   • BIOTIN enhances growth factors and cell adhesion.

5. Application

• For Skin Rejuvenation:
  • Inject PRP intradermally (microneedling/mesotherapy) or apply topically after laser treatments.
• For Hair Loss:
  • Inject PRP into the scalp (1–2cm intervals) using a 30G needle.
• Orthopedic Use:
  • Inject PRP into joints/tendons under ultrasound guidance.

6. Post-Treatment Care

• Advise the patient to:
  • Avoid sun exposure (for skin treatments).
  • Refrain from washing the area for 6–8 hours.
  • Use prescribed topical serums (if combined with PRP).

Conclusion:

Dermaheal PRP Tube ACD Gel Biotin by Nexgen Labs is a revolutionary platelet-rich plasma therapy solution, providing higher platelet recovery, increased stability, and reduced cell activation. Formulated for dermatology, orthopedics, and regenerative medicine, these CE-marked, FDA-approved PRP tubes provide accuracy, sterility, and conformity to international medical standards.
With triple-function benefits—ACD anticoagulant, GEL separator, and Biotin additive—the innovative 12ml PRP tube maximizes therapeutic gains, making it a trusted one among clinicians and healthcare professionals alike. Rely on Nexgen Labs for excellent-quality, medical-grade PRP products at wholesale rates. Transform your clinical practice with established efficacy and uncompromising quality.